Antioxidant and Antiglycation Properties of Crude Methanolic Extracts of Canola Meal, Soybean Meal and Their Composite Mixture — 104a — Nirat Katuwal and Bishnu Karki

Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA

Soybean and canola processing industries generate a substantial amount of meal as byproducts. Although these meals are good source of high-value polyphenolic compounds, research on their biological activities such as biological activities such as advanced glycation end-products (AGEs) inhibitory activities are scarce. AGEs are formed by a non-enzymatic reaction between reducing sugars and proteins and are implicated in various diseases including diabetes. The side effects of synthetic AGE inhibitors have increased research on the identification and isolation of AGE inhibitors from natural sources. Therefore, in this study, we determined the antioxidant and AGE-inhibitory activities of crude methanolic extracts of hexane-extracted canola meal (HECM), cold-pressed soybean meal (CPSM), and their composite mixtures (COM). A simplex lattice mixture design (SLMD) was used to prepare eight mixtures with fractions of individual meals ranging from 0 to 100%. The mixtures were extracted with 80% aqueous methanol and analyzed for total phenol content, total flavonoid content, antioxidant activities, and AGE inhibitory activities.

The results revealed that the crude extract of HECM exhibited higher contents of total phenol, total flavonoid, and antioxidant activities compared to CPSM. These properties increased with increasing proportions of canola meal in the mixture. Though the study is ongoing, preliminary findings suggest that both HECM and CPSM can potentially act as safe AGE inhibitors. Identifying such high-value molecules from these byproducts could be valuable to the pharmaceutical industry and potentially contribute to the valorization of these meals.

South Dakota State University
Dr Bishnu Karki